e ab f1209j  (Elabscience Biotechnology)

Journal: iScience

Article Title: Hypoxic-immune axis orchestrates metastatic dissemination via HIF isoform imbalance in pancreatic neuroendocrine tumors

doi: 10.1016/j.isci.2025.114340

Figure Lengend Snippet: MDSC subsets in KRAS-mutated PNETs (A) Representative flow cytometry plots and histogram overlays demonstrate elevated CD11b + cell proportions in KRAS G12C patient blood samples compared to wild-type KRAS tumors and healthy controls. (B) Quantification shows a slight elevation in CD14 + cell frequency compared to wild-type KRAS, alongside a marked enrichment relative to healthy controls. (C) A moderate rise in CD15 + cell frequency in KRAS G12C samples versus wild-type KRAS, with a pronounced elevation compared to normal baseline levels. (D) Statistical comparisons confirm a significant increase in CD33 + cell populations relative to both wild-type KRAS and healthy controls.

Article Snippet: PerCP/Cyanine5.5 Anti-Human CD14 Antibody [M5E2] , Elabscience , E-AB-F1209J.

Techniques: Flow Cytometry

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: NLRP3 in tumor-associated macrophages predicts a poor prognosis and promotes tumor growth in head and neck squamous cell carcinoma

doi: 10.1007/s00262-022-03357-4

Figure Lengend Snippet: NLRP3 is overexpressed in the stromal cells of human HNSCC. a Representative immunofluorescence images of NLRP3 (red) and CK-14 (green) in human HNSCC tissue. Bar, 50 μm. b Flow cytometry to detect NLRP3 expression (mean ± SD) in HNSCC TILs, and cells were gated by CD14+. c, d Representative IHC images and corresponding quantification data of NLRP3 expression in oral mucosa (n = 20), dysplasia (n = 61) and HNSCC (n = 157) (***, P < 0.001). Bar, 50 μm. e Representative IHC staining of NLRP3 in HPV-negative (n = 142) and HPV-positive HNSCC tissue (n = 15) and f quantification analysis (**, P < 0.01). Bar, 50 μm. G Kaplan–Meier analysis using overall survival (OS) of NLRP3 expression in HNSCC patients. median cut-off, n = 148. Error bar, SD

Article Snippet: PerCP-Cyanine5.5 anti-human CD14 (45-0149-42), APC anti-human CD206 (17-2069-42), PerCP-Cyanine5.5 anti-mouse F4/80 (45-4801-80), APC anti-mouse CD206 (17-2069-42), PE-Cyanine7 anti-human CD68 (25-0689-42), APC anti-mouse Ly6C (17-5932-82), PE-Cyanine7 anti-mouse Ly6G (25-9668-82) and eFluor 506 Fixable Viability Dye (65-0866-14) were from eBioscience.

Techniques: Immunofluorescence, Flow Cytometry, Expressing, Immunohistochemistry

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: NLRP3 in tumor-associated macrophages predicts a poor prognosis and promotes tumor growth in head and neck squamous cell carcinoma

doi: 10.1007/s00262-022-03357-4

Figure Lengend Snippet: Increased NLRP3 expression in TAMs predicts poor prognosis in HNSCC. a The ratio of M1 (CD64, CD68) and M2-like macrophage (MRC1, CD163) signatures in the low-expression (n = 259) and high-expression NLRP3 groups (n = 259) in the TCGA HNSCC database (***, P < 0.001). b Co-immunofluorescence staining of NLRP3 (red) and CD206 (green) in human HNSCC tissue. The white arrow shows co-expressed cell (yellow). T, tumor area; S, stromal area. Bar, 50 μm. c Flow cytometry to detect NLRP3 expression (mean ± SD) in CD206+ TAMs in human HNSCC PBMCs (n = 6) and TILs (n = 5). d Quantitative statistical analysis of NLRP3- and CD206-positive populations on gated cells (**, P < 0.01; ***, P < 0.001). e Correlation analysis of CD14+/CD206+/NLRP3+ and CD14+/CD206+ cell proportions in HNSCC patient PBMC and TIL, n = 11. f Representative IHC staining of NLRP3, CD206 and CD163 in human HNSCC using serial sections. Bar, 50 μm. g Correlation analysis between NLRP3, CD206 and CD163 protein expression in HNSCC tissue microarray using histoscore, n = 157. h Correlation analysis between NLRP3, MRC1 (CD206 encoding gene) and CD163 RNA expression in the TCGA HNSCC database. unit, expression (RSEM, Log2(Val + 1)), n = 520. i Hierarchical clustering plot of NLRP3, CD11b, CD206 and CD163 in HNSCC based on histoscore. n = 74. j Survival curves of high NLRP3 and CD206 expression vs. low NLRP3 and CD206 expression patients; high NLRP3 and CD163 expression vs. low NLRP3 and CD163 expression patients; low NLRP3 on high CD206 expression vs. high NLRP3 on CD206 high expression patients; and low NLRP3 on high CD163 expression vs. high NLRP3 on CD163 high expression patients. Error bar, SD

Article Snippet: PerCP-Cyanine5.5 anti-human CD14 (45-0149-42), APC anti-human CD206 (17-2069-42), PerCP-Cyanine5.5 anti-mouse F4/80 (45-4801-80), APC anti-mouse CD206 (17-2069-42), PE-Cyanine7 anti-human CD68 (25-0689-42), APC anti-mouse Ly6C (17-5932-82), PE-Cyanine7 anti-mouse Ly6G (25-9668-82) and eFluor 506 Fixable Viability Dye (65-0866-14) were from eBioscience.

Techniques: Expressing, Immunofluorescence, Staining, Flow Cytometry, Immunohistochemistry, Microarray, RNA Expression